Genome-wide CRISPR screening is a powerful tool for systematically identifying gene functions across the entire genome. This approach utilizes CRISPR-Cas9 technology to introduce targeted gene disruptions, allowing researchers to study genetic dependencies, disease mechanisms, and therapeutic targets. By employing large-scale sgRNA libraries, scientists can perform high-throughput loss-of-function studies, uncovering critical genes involved in various biological processes. Genome-wide CRISPR screening is widely used in cancer research, drug discovery, and functional genomics to accelerate advancements in precision medicine.